Project
Part:BBa_K153002:Design
Designed by: Dmitry O. Malyshev Group: iGEM08_University_of_Sheffield (2008-10-29)
CsrB with promoter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 49
Illegal SpeI site found at 582
Illegal PstI site found at 568 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 49
Illegal SpeI site found at 582
Illegal PstI site found at 568
Illegal NotI site found at 573 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 49
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 49
Illegal SpeI site found at 582
Illegal PstI site found at 568 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 49
Illegal SpeI site found at 582
Illegal PstI site found at 568 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 498
Design Notes
In the natural E.Coli system CsrA is an important gene, the product of which inhibits many genes responsible for glycogen formation, gluconeogenesis, and biofilm formation. During a quorum sensing event, it is in turn inhibited by CsrB, thus allowing biofilm formation.
Source
to be added